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Plant Biotechnology

The Plant Biotechnology Laboratory specializes in all aspects of plant tissue culture with emphasis on the genetic transformation of crop plants using Agrobacterium tumefaciens. The Laboratory is particularly skilled at the transformation of potato and alfalfa and the subsequent analysis of transgenic plants. Most of the laboratory's funding is from competitive federal research grants. Plant tissue culture and molecular biology services are available and the staff welcomes collaborative or contract research projects with academic and industrial users.

For more information, or to request services, contact:

Sandra Austin-Phillips, Ph.D. 608/262-7293
e-mail: saustinp@facstaff.wisc.edu
Fax: 608/262-6748

Research Interests

Crop plants as bioreactors
Current research focuses on a multidisciplinary feasibility study for producing industrial enzymes in transgenic alfalfa i.e. using alfalfa as an animal feed bioreactor. This involves molecular approaches to maximize foreign protein expression and methods development for the extraction and purification of commercial proteins from field- grown transgenic alfalfa.


Protoplast fusion to produce somatic hybrids
Scientists in the Laboratory use protoplast fusion techniques to transfer disease and pest resistance from sexually incompatible wild Solanum and Lycopersicon species into potato breeding lines.

Services

Services are primarily custom research projects ranging from micropropagation to plant transformation. Contract research can be performed on an hourly basis or arranged as a long-term project.

Rates inclusive of supplies:

Plant tissue culture services $40/hour
Molecular biology services $50/hour