Chemistry 638: Introduction to Mass Spectrometry

1 credit: Spring 2008 - Thursdays 9:55 am, Chemistry Room 2311

Instructors: Dr. Martha M. Vestling and Dr. Amy C. Harms

Mass spectrometry has evolved into one of the leading technologies for biological and chemical research. It is widely used in genomics, proteomics, drug discovery, forensics, polymer characterization, and combinatorial chemistry.

This course is an opportunity for you to learn more about this exciting technology.

Topics to be covered in this course:

basic vocabulary of mass spectrometry
ionization options and instrument types
basic sample handling, common contaminants
databases
fragmentation and sequencing
separations before mass spectrometry: LC/MS, GC/MS
peptides and proteins, post-translational modifications
when to use stable isotopes
metabolite identification
quantitative analysis

Lectures will mix the basics of mass spectrometry with examples of sophisticated applications.

Grades will be based on attendance and a short paper.

Text: "The Expanding Role of Mass Spectrometry in Biotechnology," 2nd ed., Gary R. Siuzdak, MCC Press, San Diego, 2006.