
Agilent
2100 Bioanalyzer:
The Agilent 2100 BioAnalyzer
utilizes "Lab-on-a-Chip" technology to integrate sample preparation,
fluid handling, separation, detection, and data analysis. Agilent Technologies has Lab-on-a-Chip kits available that analyze RNA, DNA,
and Proteins.
Some examples of analysis
that can be done are:
• Quantitative analysis of PCR fragments
• Restriction fragment sizing
• Cy 5 labeled cRNA and cDNA
• RNA analysis up to 6,000 bp
• DNA analysis from 25 - 12,000 bp
• Analyze protein samples (cell lysate, column factions,
and purified proteins) ranging
in size from 14 - 200 kDa.
Affymetrix recommends the
2100 BioAnalyzer for qualifying total (eukaryotic and prokaryotic) or
messenger RNA.
Individuals must be trained
satisfactorily by a Center Specialist prior to gaining access to the instrumentation. There is a one time training fee of $50.00 per hour. The chip cost is not included in the cost of the training. A maximum of 4 people maybe trained at one time. Please contact the GEC at
gecinfo@biotech.wisc.edu
to schedule training.
Price includes the Agilent Chip, all consumables, reagents
and RNA ladder.
If a lab is planning on running a large number
of samples, it is recommended you purchase your own kit.
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