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GEC NimbleGen Services/Roche LightCycler/ UW NimbleGen Contract Services
Affymetrix GeneChip® Services
One Round Amplification 3' Expression Target Labeling
Two Round Amplification aRNA 3'Expression Target Labeling
Whole Transcript Target Labeling Service
3' Expression Hybridization Service
Whole Transcript Sense Target Hybridization Service
The prices listed here are for UW-Researchers only
All prices are effective May 01, 2009
3' Expression Target Labeling Service
One Round Amplification 3' Expression Target Labeling
Using Ambion's MessageAmp™ II-Biotin Enhanced Labeling Kit up to 12 samples can be processed at one time. The recommended total RNA in-put is 1000ng with 100ng being the minimum. GEC staff will qc all samples prior to start of labeling the assays using the Agilent 2100 BioAnalyzer to verify sample integrity and the NanoDrop Spectrophotometer to verify sample concentration and purity.
Please refer to the Guidelines for Sample Submission sheet prior to bringing your samples to the GEC. When you bring over your samples please fill out a sample drop off form, and insure your samples are legibly labeled with a sample name and your P.I.'s name.
| Sample setup and labor fee (1-12 samples) | $375.00 |
| Target Labeling fee for Sample 1 - 2 (per sample) | $90.00 |
| Target Labeling fee for Sample 3-12 (per sample) | $60.00 |
Two Round Amplification aRNA 3'Expression Target Labeling
Using Ambion's MessageAmp™ II aRNA Amplification and Labeling Kit up to 12 samples can be processed at one time.The recommended total RNA in-put 100ng with 0.1ng being the minimum. GEC staff will qc all samples prior to start of labeling assays using the Agilent 2100 BioAnalyzer to verify sample integrity and the NanoDrop Spectrophotometer to verify sample concentration and purity.
Please refer to the Guidelines for Sample Submission sheet prior to bringing your samples to the GEC. When you bring over your samples please fill out a sample drop off form, and insure your samples are legibly labeled with a sample name and your P.I.'s name.
| Sample setup and labor fee (1-12 samples) | $600.00 |
| Target Labeling fee for Sample 1 - 2 (per sample) | $145.00 |
| Target Labeling fee for Sample 3-12 (per sample) | $116.00 |
Whole Transcript Target Labeling Service
Using Affymetrix's GeneChip® Whole Transcript (WT) Sense Target Labeling kit up to 12 samples can be processed at one time. This assay is optimized specifically for use with the GeneChip® ST Arrays where "ST" stands for "Sense Target," and where the probes on the arrays have been selected to be distributed throughout the entire length of each transcript. This assay has two configurations with the key differences indicated in the following table.
Two Configurations of the WT Sense Target Labeling Assay
(Affymetrix WT Sense Target Manual)
| Protocol | 1 μg Total RNA | 100 ng Total RNA |
| Recommended Amount of Starting | 1 μg Total RNA | 100 ng Total RNA |
| Acceptable Range of Input Amount | 1-3 ug | 100-300ng |
| Procedural Difference | *Requires rRNA Reduction with RiboMinus Kit (Invitrogen) | Omits rRNA Reduction |
| Exon ST Arrays | Recommended | Not Optimal |
| Gene ST Arrays | Acceptable | Recommended |
* rRNA Reduction is done by user. Affymetrix recommends RiboMinus Human/Mouse Transcriptome Isolation kit from Invitrogen.
| Sample setup and labor fee (1-12 samples) | $600.00 |
| Target Labeling fee for Sample 1 - 2 (per sample) | $171.00 |
Target Labeling fee for Sample 3-12 (per sample) |
$142.00 |
Prokaryotic Labeling
Please contact the GEC for pricing.
Affymetrix GeneChip® Hybridization Service
3' Expression Hybridization Service
Following Affymetrix recommended protocols and procedures, biotin-labeled cRNA is hybridized to the appropriate GeneChip® overnight in the AFX HybOven480, post processed on the automated Fluidics450 Station, and scanned on the GC 3000 G7 Scanner. Data is extracted from the scanned image using AFX Expression Console software. An Excel spreadsheet of the extracted data is provided along with all files generated on the Affymetrix GeneChip® System. User is notified by e-mail that their data and project summary is available on the UWBC file server for their downloading convenience.
Please refer to the Guidelines for Sample Submission sheet prior to bringing your samples to the GEC. When you bring over your samples please fill out a sample drop off form, and insure your samples are legibly labeled with a sample name and your P.I.'s name.
| GeneChip® setup and labor fee (1-4 arrays) | $225.00 |
| GeneChip® setup and labor fee (5-12 arrays) | $400.00 |
| GeneChip® Hybridization & Post Processing fee per array | $75.00 |
Researchers are responsible for purchasing all GeneChips®. Please refer to the current UW-Madison Academic Pricing Sheet for all GeneChip® prices. To ensure academic pricing,when ordering from Affymetrix (1-888-362-2447), please refer to UW Contract #10-5540.
Whole Transcript Sense Target Hybridization Service
Following Affymetrix recommended protocols and procedures, end-terminus biotin labeled DNA is hybridized to the appropriate GeneChip® overnight in the AFX HybOven480, post processed on the automated Fluidics450 Station, and scanned on the GC 3000 G7 Scanner. Data is extracted from the scanned image using AFX Expression Console software. An Excel spreadsheet of the extracted data is provided along with all files generated on the Affymetrix GeneChip® System. User is notified by e-mail that their data and project summary is available on the UWBC file server for their downloading convenience.
Please refer to the Guidelines for Sample Submission sheet prior to bringing your samples to the GEC. When you bring over your samples please fill out a sample drop off form, and insure your samples are legibly labeled with a sample name and your P.I.'s name.
| GeneChip® setup and labor fee (1-4 arrays) | $225.00 |
| GeneChip® setup and labor fee (5-12 arrays) | $400.00 |
| GeneChip® Hybridization & Post Processing fee per array | $75.00 |
Researchers are responsible for purchasing all GeneChips®. Please refer to the current UW-Madison Academic Pricing Sheet for all GeneChip® prices. To ensure academic pricing, when ordering from Affymetrix (1-888-362-2447), please refer to UW Contract
#10-5540.
Please note: No refund for poor results. If a GeneChip® appears faulty at time of hybridization and/or analysis, a new GeneChip® will be requested from Affymetrix, but Center charges still apply.
The NetAffx web site is available for searching GeneChip® sequence information and for correlating array results with public and proprietary databases http://www.affymetrix.com/analysis/index.affx
For additional information about the availability of Affymetrix GeneChips® please see:
www.affymetrix.com/products/products_list.html
Non-UW resesearchers please contact the GEC for a price quote
UW-Madison Affymetrix Sales Representatives is Todd Finch 1-800-223-5281
UW-Madison Field Applications Specialist is Christine Ramos 1-800-223-5281 ext. 4252